The Mass Spectrometry society of Japan - The 68th Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lectures

Award Lecture

Oral Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Panel Discussion

Luncheon Seminars (Day1, Day2, Day3)

Corporate Program

Poster Presentations

Day 3, June 24（Fri.）　　Room P (501, 502 and 503)

3P-20　　PDF

Characterization of Intact mRNA Using IP-RP-TOF-MS, SEC-MALS, and CDMS

(1Nihon Waters, 2Waters - Milford, 3Megadalton)
oKenji Hirose1, Maki Terasaki1, Patrick Brophy2, Shion Henry2, Benjamin Draper3, Daniel Botamanenko3, Ying-Qing Yu2, Martin Jarrold3

There is a pressing need to analyze intact mRNA that has been accelerated by the recent development of mRNA-based vaccines in response to the COVID-19 pandemic. mRNA production by enzymatic in vitro transcription followed by enzymatic or chemical capping can produce a variety of reaction products. Unwanted products and contaminants such as double-stranded RNA (dsRNA), truncated RNA fragments, and heterogeneity in the poly(A) tail, and degradation products can be better characterized by examining intact mass distributions. Herein, we demonstrate intact mass analysis of mRNA using a variety of conventional techniques with chromatographic separation (IP-RP/TOF-MS & SEC-MALS). Additionally, we analyze mRNA by charge detection mass spectrometry (CDMS) and compare these results to those obtained by the more conventional methods.