The Mass Spectrometry society of Japan - The 68th Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lectures

Award Lecture

Oral Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Panel Discussion

Luncheon Seminars (Day1, Day2, Day3)

Corporate Program

Poster Presentations

Day 3, June 24（Fri.）　　Room P (501, 502 and 503)

3P-16　　PDF

I n process LC MS media monitoring for Ambr® 15 Cell Culture high throughput automated bioreactor system

(1Nihon Waters, 2Waters - Milford, 3Sartorius)
oRyotaro Hirabayashi1, Kenji Hirose1, Prochaska Charles3, Alelyunas Yun2, Kukla Clint3, Wrona Mark2

Cell culture media provides essential nutrients and components required to sustain cell health and growth and for maximizing biotherapeutic production and quality. There is increasing interest from bioprocess groups to monitor raw media feed, feed supplements and spent cell culture media. In addition to essential amino acids, vitamins and other primary feed components, the spent media contains hundreds of compounds. Their monitoring could provide additional information about the performance of the bioreactor experiments during clone selection and/or process optimization. For this work, we will discuss a LC-MS based routine screening of in-process samples from a high throughput bioreactor system.