The Mass Spectrometry society of Japan - The 68th Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lectures

Award Lecture

Oral Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Panel Discussion

Luncheon Seminars (Day1, Day2, Day3)

Corporate Program

Poster Presentations

Day 3, June 24（Fri.）　　Room P (501, 502 and 503)

3P-14　　PDF

Nucleic acid analysis using MALDI-DIT-MS

(Shimadzu)
oYuko Fukuyama, Hideharu Shichi, Masaki Murase, Yoshihiro Yamada, Sadanori Sekiya, Shinichi Iwamoto, Koichi Tanaka

Recently, practical application of nucleic acid medicines has rapidly advanced, and the importance of nucleic acid analytical technique has increased. However, reports of nucleic acid analysis by matrix-assisted laser desorption/ionization (MALDI) method have been limited. In this study, we attempted to develop a nucleic acid analysis method using MALDI-digital ion trap-mass spectrometer (MALDI-DIT-MS). Nucleic acid of MW 6201 was detected with high sensitivity as [M+H]+ using 3-hydroxypicolinic acid as a matrix and ammonium citrate as an additive. In-source decay (ISD) of the nucleic acid was improved by optimizing equipment condition and matrix. Sequencing analysis was accelerated by in-house software. Finally, rapid molecular weight analysis and sequence analysis including modification site were achieved for nucleic acid medicines after desalting purification.