The Mass Spectrometry society of Japan - The 68th Annual Conference on Mass Spectrometry, Japan

Abstract

Poster Presentations

Day 3, June 24(Fri.)  Room P (501, 502 and 503)

13C-tracer Analysis of Phospholipids by LC-QTOF/MS

(1Osaka Univ., 2Osaka Univ., 3Osaka Univ., 4Shimadzu)
oYuki Yamada1, Keita Murakami1, Nobuyuki Okahashi1,2,3, Atsuhiko Toyama4, Junko Iida2,4, Fumio Matsuda1,2,3

A 13C-tracer analysis using mass spectrometry was developed for investigating dynamics of lipid metabolism in budding yeast Saccharomyces cerevisiae, a model eukaryote organism. Labeling ratio of 9 phospholipids and glycerolipids (PA, PG, PS, PC, PE, PI, DAG, TAG and CL) was determined based on the incorporation of [U-13C]glucose using LC-QTOF/MS.