The Mass Spectrometry society of Japan - The 68th Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lectures

Award Lecture

Oral Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Panel Discussion

Luncheon Seminars (Day1, Day2, Day3)

Corporate Program

Poster Presentations

Day 3, June 24（Fri.）　　Room P (501, 502 and 503)

3P-06　　PDF

Development of highly sensitive single-cell mass spectrometry for amino acids in living cells

(1Univ. Shizuoka, 2Ajinomoto)
oHajime Mizuno1, Kimika Nishijima1, Yukino Yamaguchi1, Sachise Karakawa2, Masashi Harada2, Eiji Sugiyama1, Akira Nakayama2, Kenichiro Todoroki1

In this study, we developed a highly sensitive analytical method by introducing a derivatization method to live single-cell mass spectrometry to detect intracellular amino acids. Trace amounts of amino acid standards were derivatized with (R)-BiAC reagent in a glass capillary for nanoESI, and mass spectrometric analysis showed improved peak intensities for all amino acids. Furthermore, this method was applied to single-cell analysis, and derivative peaks of all 20 amino acids in the cell could be detected at the single-cell level.