The Mass Spectrometry society of Japan - The 68th Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lectures

Award Lecture

Oral Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Panel Discussion

Luncheon Seminars (Day1, Day2, Day3)

Corporate Program

Poster Presentations

Day 2, June 23（Thu.）　　Room P (501, 502 and 503)

2P-42（2D-O1-1045）　　PDF

Quantitative evaluation of LC/MS methods for blood metabolomic data integration

(1Kyushu Univ., 2Kyushu Univ., 3JSPS)
oYuri Imado1, Masatomo Takahashi2, Yuki Soma1,2, Shunsuke Aburaya2,3, Kohta Nakatani2, Yoshihiro Izumi1,2, Takeshi Bamba1,2

Metabolomics, mass spectrometry-based comprehensive analysis of metabolome, has attracted attention as an analytical approach to provide novel insight into metabolism and to search for biomarkers. However, one of the current problems in the field of metabolomics is the difficulty in integrating data collected using different equipment at different facilities, because many metabolomic methods have been developed independently and are unique to each laboratory. Herein, we evaluated the quantitative performance of four types of liquid chromatography (LC), including hydrophilic-interaction LC (HILIC), ion chromatography with an anion exchange column (AEX), LC with pentafluorophenylpropyl column (PFPP-LC), and unified HILIC/AEX, coupled with benchtop quadrupole Orbitrap high-resolution mass spectrometry for hydrophilic metabolome analysis of human plasma.