Oral Sessions (Day1, Day2, Day3)
Poster Presentations (Day1, Day2, Day3)
Poster Presentations
- Day 2, June 23（Thu.） Room P (501, 502 and 503)
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2P-22 PDF
Three-minute lipidomics using ion mobility spectrometry
The usefulness of detailed information on lipid metabolites in human blood samples as biomarkers for disease risk and diagnosis is becoming increasingly clear. Addressing this demand, the field of lipidomics is increasingly focusing on large-scale, comprehensive analysis of lipids.
Throughput for multiple sample analysis as well as reproducibility of the analysis over a long period of time are important for the lipidomics of human blood samples. Therefore, we have developed a rapid measurement method that significantly reduces analysis time, and operated the system with emphasis on reproducibility by utilizing QC (quality control) samples. While improving throughput requires minimizing measurement time, reducing chromatographic analysis time results in fewer lipids detected in LC/MS. To overcome this issue, we have employed an ion mobility separation axis to the conventional LC/MS and constructed a rapid analysis system that can measure one sample in three minutes. This enabled measurement of more than 400 samples per day, and also facilitated frequent measurement of QC samples and repeated measurement of the same samples to obtain reliable data sets. This presentation discusses the actual data obtained from this LC/IMS/MS analysis and the resulting observations from the analysis of a set of Alzheimer's disease samples