The Mass Spectrometry society of Japan - The 68th Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lectures

Award Lecture

Oral Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Panel Discussion

Luncheon Seminars (Day1, Day2, Day3)

Corporate Program

Poster Presentations

Day 2, June 23（Thu.）　　Room P (501, 502 and 503)

2P-19　　PDF

Development of Plasma Neurogranin Assay using IP-MALDI-MS

(Shimadzu)
oNaoki Kaneko, Ryota Takahashi, Akihiro Korenaga, Ritsuko Yoda, Sadanori Sekiya, Shinichi Iwamoto, Koichi Tanaka

Neurogranin (Ng) is a cerebrospinal fluid (CSF) biomarker for synaptic dysfunction at Alzheimer’s disease (AD). The clinical significance of Ng peptides in plasma has remained unclear. In this study, we developed an immunoprecipitation combined with matrix-assisted laser desorption ionization time-of-flight mass spectrometry (IP-MALDI-MS) for Ng peptides. Some peaks corresponding to a variety of Ng peptides and proteins were detected in a plasma sample. The accuracy in linear regression model showed within 80-120% in three Ng peptides. Intra assay precision of Ng peptides in plasma was less than 20%CV. IP-MALDI-MS enable the highly sensitive and simultaneous semi-quantitative measurement of multiple Ng peptides levels. It could be helpful in the analysis of plasma Ng peptides for AD biomarker.