The Mass Spectrometry society of Japan - The 68th Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lectures

Award Lecture

Oral Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Panel Discussion

Luncheon Seminars (Day1, Day2, Day3)

Corporate Program

Poster Presentations

Day 2, June 23（Thu.）　　Room P (501, 502 and 503)

2P-03　　PDF

Lipid Profiling of Abcd1 knockout mouse brain by lipidomics

(1Mitsubishi Tanabe Pharm., 2Toyama Univ.)
oTomoya Akashi1, Masayoshi Saito1, Naoki Fujitani1,2, Kozo Oka1, Masashi Morita2

X-linked adrenoleukodystrophy (X-ALD) is a genetic disorder caused by mutations in genes encoding peroxisomal Abcd1 protein. Functional deficiency of Abcd1 cause the accumulation of very long chain fatty acid (VLCFA) in the body, which result in the demyelination of the central nervous system and adrenal insufficiency. Although total VLCFA levels in blood have been used to diagnose X-ALD, they do not correlate with its clinical CNS symptoms, and it is not clear which lipid classes is involved in the pathogenesis. In the present study, we performed a comprehensive lipidomics analysis of lipids in the brain of Abcd1 knockout mice, an animal model of X-ALD, to obtain profiling data for the identification of potent biomarkers that correlate with the disease symptoms. Total lipids in the brains of Abcd1 knockout and wild-type mice were extracted using the Bligh-Dyer method, and lipidomic analysis was performed using liquid chromatography-triple quadrupole mass spectrometry (LC-TQMS) and -Orbitrap mass spectrometry (LC-Orbitrap MS). In addition, we performed quantitative analysis of the most promising biomarker candidates by LC-TQMS.