Abstract

Poster Presentations

Day 2, June 23（Thu.）　　Room P (501, 502 and 503)

2P-01　　PDF

Pharmacokinetic Analysis of Small Drugs by Mass Spectrometry Imaging Combined with Product-Ion Analysis

(¹HUSM, ²Preppers Co. Ltd., ³Shimadzu Corporation, ⁴Shimadzu Scientific Instruments)
^oAriful Islam¹, Yutaka Takahashi^1,2, Takumi Sakamoto¹, Shuhei Aramaki¹, Md. Mamun¹, Koretsugu Ogata³, M. Boutaghou⁴, Tomoaki Kahyo¹, Mitsutoshi Setou^1,2

Comprehensive information of administered drugs in biological tissues is of paramount importance to understand their clinically-relevant properties, efficacy, potential side effects, and toxicity. Mass spectrometry imaging (MSI) is a label-free and highly sensitive imaging technique that offers accurate visualization of drugs and their metabolites in the tissue sections. Matrix-assisted laser desorption/ionization MSI (MALDI MSI) is commonly used for the mapping of analytes present in the sample. In this study, we are applying MALDI-MSI (iMScope QT, Shimadzu, Japan) for pharmacokinetics analysis of small drugs, including imipramine, and chloroquine in wild-type mice. We have tested several samples by iMScope QT to confirm data acquisition speed with a higher spatial resolution (up to 5 μm). Now we are trying to develop MALDI-MSI methods for the detection and product-ion analysis of imipramine, chloroquine, and their metabolites in tissue samples. After optimizing methods, we will inject those drugs intraperitoneally into mice. At different time points of the injection of those drugs, all major organs of mice will be collected and will be used for the pharmacokinetic analysis of those drugs applying iMScope QT. We expect that our method applying MSI will offer detailed spatial information of drugs and their metabolites.