Oral Sessions (Day1, Day2, Day3)
Poster Presentations (Day1, Day2, Day3)
Poster Presentations
- Day 1, June 22（Wed.） Room P (501, 502 and 503)
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1P-38 PDF
Development of High-Sensitivity/High-Throughput Proteomics Systems for Minute Sample Analysis
Shotgun proteomics using nano liquid chromatography/tandem mass spectrometry typically involves low flow rates of less than 1 µL/min. However, such conditions require a long time for column washing and equilibration, which is a major obstacle when performing high throughput measurements. Therefore, we constructed a high-speed LC/IMS/MS system using capillary LC, and succeeded in reducing the measurement time from 2 hours to 2 minutes. On the other hand, the use of capillary LC at several µL/min reduces sensitivity compared to nano LC, which may make it difficult to apply to minute samples. In this study, we measured minute samples using the high throughput capillary LC/IMS/MS/MS system and found that an average of 2,235 protein groups were identified for a 250 ng sample, whereas 111 protein groups were identified for a 0.5 ng sample. In this presentation, results using a hydrophilic interaction chromatography (HILIC) column will also be reported.