The Mass Spectrometry society of Japan - The 68th Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

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Award Lecture

Oral Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

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Day 1, June 22（Wed.）　　Room P (501, 502 and 503)

1P-35　　PDF

N-Glycoproteomics of plasma proteins from semi-supercentenarians using sialic acid linkage-specific derivatization

(1TMIG, 2Shimadzu, 3Keio Univ., 4Osaka Univ.)
oHiroki Tsumoto1, Takashi Nishikaze2, Yasumichi Arai3, Kazunori Ikebe4, Tatsuro Ishizaki1, Kei Kamide4, Yasuyuki Gondo4, Yukie Masui1, Keitaro Umezawa1, Kyojiro Kawakami1, Nobuyoshi Hirose3, Yoshihiro Hayakawa2, Yuri Miura1, Tamao Endo1

Semi-supercentenarians (SSCs, older than 105 years) are a good example of human longevity, therefore physiological and biochemical analyses of SSCs can contribute to the understanding of healthy human aging. In this study, we performed N-glycoproteomics of plasma proteins obtained from SSCs and younger control groups using a combination of LC-MS/MS and sialic acid linkage-specific alkyl amidation (SALSA) method to discriminate between α2,3- and α2,6-sialic acid. As a result of LC-MS/MS analyses, 1676 N-glycopeptides from 91 proteins were identified and quantified. Among them, 366 N-glycopeptides were altered in SSCs compared to younger control groups. Furthermore, multivariate analysis identified 10 N-glycopeptides which were characteristic of SSCs. Most of the identified N-glycopeptides were derived from α1-acid glycoprotein 1.