Oral Sessions (Day1, Day2, Day3)
Poster Presentations (Day1, Day2, Day3)
Poster Presentations
- Day 1, June 22（Wed.） Room P (501, 502 and 503)
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1P-30 PDF
Development of an ultra-comprehensive protein quantitative method using human induced pluripotent stem cells (hiPSCs) and its application to glycosyltransferase analysis
Protein glycosylation is one of the major post-translational modifications. Quantitative studies of glycosyltransferases have a great impact on understanding biological function of glycosylation. The data-independent LC/MS/MS (DIA-MS) method is a powerful tool for quantification of proteins; however, the DIA-MS requires a comprehensive library, including low-abundance glycosyltransferases. In this study, we performed 1) a sample preparation to create an ultra-comprehensive protein library using hiPSCs, and 2) quantitative analysis of glycosyltransferases during differentiation of hiPSCs into neural stem cells by DIA-MS. We found the changes in the levels of several glycosyltransferases associated with O-glycosylation.