The Mass Spectrometry society of Japan - The 68th Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lectures

Award Lecture

Oral Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Panel Discussion

Luncheon Seminars (Day1, Day2, Day3)

Corporate Program

Poster Presentations

Day 1, June 22（Wed.）　　Room P (501, 502 and 503)

1P-09　　PDF

Leveraging a higher duty cycle DIA acquisition on a novel QTOF for enhanced proteomics analysis

(Sciex)
oUshio Takeda

The ability to identify and quantify large number of proteins and peptides is of great importance in translational medicine and life science research. Data independent acquisition (DIA) approaches have been shown to surpass data dependent acquisition (DDA) methodologies in terms of protein identifications in complex matrices especially at shorter acquisition speeds. Our new QTOF system equipped with a novel Zeno trap(1 is able to deliver sensitivity gains in variable window SWATH acquisition. The built-in Zeno trap increases duty cycle at MS/MS level to over 90%, allowing for unprecedented gains in sensitivity (5-20x) at MS/MS, resulting in more identifications using Zeno DIA acquisition. We evaluated increases in protein and peptide identifications using SWATH acquisition vs. Zeno DIA at various EvoSep sample throughputs.