Abstract

Poster Presentations

Day 1, June 22（Wed.）　　Room P (501, 502 and 503)

1P-07　　PDF

Peptide sequence analysis using MS² of alkali metal ion adducts

(¹Hiroshima Univ., ²Shimadzu)
Yohei Saito¹, ^oManaho Yamaguchi¹, Yuko Fukuyama², Koichi Tanaka², Shunsuke Izumi¹

In matrix-assisted laser desorption/ionization (MALDI), peptides are often detected as [M+H]⁺, but they can also be observed as alkali metal adducts, such as [M+Na]⁺. In this study, MS² of alkali metal ion adducts ([M+Li]⁺, [M+Na]⁺, [M+K]⁺, [M+Rb]⁺ and [M+Cs]⁺) and [M+H]⁺ for peptides were compared. As a result, MS² fragments for all the alkali metal ion adducts were mainly detected as y-ions or b-ions. In particular, ratio of total peak intensity of y-ions to total peak intensity of b-ions was highest in the [M+Cs]⁺ MS² spectrum.