The Mass Spectrometry society of Japan - The 68th Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lectures

Award Lecture

Oral Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Panel Discussion

Luncheon Seminars (Day1, Day2, Day3)

Corporate Program

Oral Sessions

Day 1, June 22（Wed.）　18:10-18:30　Room A (Main Hall)

1A-O1-1810　　PDF

Peptidomics based on high-efficiency peptide extraction method.

(1Kitasato Univ., 2Kitasato Univ.)
oYoshio Kodera1,2

The differential solubilization (DS) method that we developed enables high-efficiency extraction of peptides from serum/plasma, including peptides bound to carrier proteins such as albumin. Using this method, we directly identified an authentic peptide hormone using LC-MS and various novel bioactive peptides. Furthermore, we modified the DS method to make it applicable to tissue samples. The modified DS method achieved highly efficient and reproducible peptide extraction from a few milligrams of frozen tissue. Subsequent LC-MS/MS analysis successfully resulted in the identification of numerous authentic bioactive peptides and a novel bioactive peptide from animal tissues. The method based on our peptide extraction technique may facilitate the discovery of novel bioactive peptides and peptides that are related to disease.