The Mass Spectrometry society of Japan - The 68th Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lectures

Award Lecture

Oral Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Panel Discussion

Luncheon Seminars (Day1, Day2, Day3)

Corporate Program

Oral Sessions

Day 1, June 22（Wed.）　16:50-17:10　Room A (Main Hall)

1A-O1-1650　　PDF

Development of Ultra-Fast Proteomics using LC-Quadrupole-Orbitrap MS with Data-Independent Acquisition

(1Kazusa DNA Res. Inst., 2Ochanomizu Univ.)
oMasaki Ishikawa1, Ryo Konno1, Daisuke Nakajima1, Mari Gotoh2, Keiko Fukasawa2, Hironori Sato1, Ren Nakamura1, Osamu Ohara1, Yusuke Kawashima1

Proteomics has become an increasingly important tool in medical and medicinal applications. It is necessary to improve the analytical throughput for these applications, particularly in large-scale drug screening to enable measurement of a large number of samples. In this study, we aimed to establish an ultrafast proteomic method based on a 5-min gradient LC and quadrupole-Orbitrap mass spectrometer (Q-Orbitrap MS). We precisely evaluated data-independent acquisition (DIA) parameters for 5-min gradient LC and reached a depth of >5,000 and 4,700 proteins from 1,000 and 250 ng HEK293T cell digest in a single-shot run, respectively. The throughput of our method enabled the measurement of approximately 80 samples/day, including sample loading, column equilibration, and wash running time. We demonstrated that our method is applicable for the screening of chemical responsivity via a cell stimulation assay. These data show that our method enables the capture of biological alterations in proteomic profiles with high sensitivity, suggesting the possibility of large-scale screening of chemical responsivity.