The Mass Spectrometry society of Japan - The 68th Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lectures

Award Lecture

Oral Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Panel Discussion

Luncheon Seminars (Day1, Day2, Day3)

Corporate Program

Oral Sessions

Day 1, June 22（Wed.）　16:30-16:50　Room A (Main Hall)

1A-O1-1630　　PDF

Adding New Dimension to Peptide Separation with Ion Mobility Spectrometry

(1Kyoto Univ., 2Univ. of Manitoba, 3NIBIOHN)
oKosuke Ogata1, Chih-hsiang Chang1, Darien Yeung2, Victor Spicer2, Oleg Krokhin2, Yasushi Ishihama1,3

Trapped ion mobility spectrometry (TIMS) provides a new ion mobility separation axis in addition to retention time and m/z in shotgun proteomics experiments. In phosphoproteomics, TIMS separations have enabled more reliable identification and quantification of positional isomers. In isobaric quantification using tandem mass tag (TMT), TIMS can be used to reduce co-eluting ions by an average of 7.6%. Furthermore, the contribution of peptide amino-acid sequence to CCS has been investigated using a dataset of ~134,000 peptides analyzed by TIMS. In addition, we systematically characterized the CCS values of 4,433 pairs of mono-phosphopeptide and corresponding unphosphorylated peptide ions and found nearly one- third of the mono-phosphopeptide ions showed smaller CCS values than their unphosphorylated counterparts.