演題概要

ポスター発表

第3日　6月24日（金）　　P会場（501，502，503）

3P-20　　PDF

イオンペア逆相LC-MS、SEC-MALSおよびCDMSを用いたインタクトmRNAの分析

(¹日本ウォーターズ・ ²Waters - Milford・ ³メガダルトン)
^o廣瀬賢治¹・ 寺崎真樹¹・ Brophy, Patrick²・ Henry, Shion²・ Draper, Benjamin³・ Botamanenko, Daniel³・ Yu, Ying-Qing²・ Jarrold, Martin³

There is a pressing need to analyze intact mRNA that has been accelerated by the recent development of mRNA-based vaccines in response to the COVID-19 pandemic. mRNA production by enzymatic in vitro transcription followed by enzymatic or chemical capping can produce a variety of reaction products. Unwanted products and contaminants such as double-stranded RNA (dsRNA), truncated RNA fragments, and heterogeneity in the poly(A) tail, and degradation products can be better characterized by examining intact mass distributions. Herein, we demonstrate intact mass analysis of mRNA using a variety of conventional techniques with chromatographic separation (IP-RP/TOF-MS & SEC-MALS). Additionally, we analyze mRNA by charge detection mass spectrometry (CDMS) and compare these results to those obtained by the more conventional methods.