- 第3日 6月24日（金） P会場（501，502，503）
Recently, practical application of nucleic acid medicines has rapidly advanced, and the importance of nucleic acid analytical technique has increased. However, reports of nucleic acid analysis by matrix-assisted laser desorption/ionization (MALDI) method have been limited. In this study, we attempted to develop a nucleic acid analysis method using MALDI-digital ion trap-mass spectrometer (MALDI-DIT-MS). Nucleic acid of MW 6201 was detected with high sensitivity as [M+H]+ using 3-hydroxypicolinic acid as a matrix and ammonium citrate as an additive. In-source decay (ISD) of the nucleic acid was improved by optimizing equipment condition and matrix. Sequencing analysis was accelerated by in-house software. Finally, rapid molecular weight analysis and sequence analysis including modification site were achieved for nucleic acid medicines after desalting purification.