演題概要

ポスター発表

第3日　6月24日（金）　　P会場（501，502，503）

3P-12　　PDF

合成オリゴヌクレオチドの配列確認と不純物分析向け新規ソフトウェア：CONFIRM Sequence

(¹日本ウォーターズ・ ²ウォーターズ)
^o寺崎真樹¹・ 矢田絵都子¹・ 平林凌太朗¹・ Doneanu, Catalin²・ Gorton, Matthew²・ Knowles, Chris²・ Morns, Ian²・ Preston, Chris²・ Reah, Ian²・ Broughton, Mike²・ Jones, Simon²・ Rewcastle, Jonathan²・ Mullins, Rory²・ Edvinas, Karmonas²・ Henesey, Paul²・ David, Townsley²・ Jackson, Dave²・ Reay, Pete²・ Hope, Gavin²・ Harry, Emma²・ Fox, Jonathan²・ Riley, Jo-Anne²・ Shion, Henry²・ Fredette, Joe²・ Yu, Ying Qing²・ 廣瀬賢治¹

Synthetic oligonucleotides have emerged in recent years as a powerful alternative to small molecule and protein therapeutics. Manufacturing and quality control of oligonucleotide therapeutics requires highly selective and sensitive LC-MS methods for impurity sequencing and quantification. The most often used mass spectrometry-based method for oligonucleotide analysis has been reversed-phase chromatography employing a variety of ion-pairing reagents and modifiers in negative ESI-MS mode (IP-RP LC-MS). One critical step for identification of oligonucleotide impurities is mass spectrometry based sequencing and data interpretation. Here we introduce a novel software suite – CONFIRM Sequence, developed for fast processing of both MS/MS and MS^E (no specific precursor selection) mass spectra recorded by IP-RP LC-MS for a chemically modified oligonucleotides and a variety of impurities.