演題概要

ポスター発表

第3日　6月24日（金）　　P会場（501，502，503）

3P-09　　PDF

Data-independent acquisiton (DIA) 法を用いたグリセロリン脂質構造異性体の定量

(¹阪大院情報・ ²阪大島津協働研・ ³阪大先導研・ ⁴島津)
^o原大樹¹・ 小森柊花¹・ 岡橋伸幸^1,2,3・ 遠山敦彦⁴・ 飯田順子^2,4・ 松田史生^1,2,3

The data-independent acquisition (DIA) mode of tandem mass spectrometry was applied for the separation of structural isomers of glycerophospholipids in Saccharomyces cerevisiae and human plasma. The parameters of DIA were optimized by a performance comparison with the data-dependent acquisition mode. Separation and relative quantification of co-eluting structural isomers was achieved by the detection of specific fragment ions from the DIA data.