- 第3日 6月24日（金） P会場（501，502，503）
Glutathione (GSH) and its metabolites play an important role in oxidoreduction homeostasis and oxidative stress tolerance1). Glutathione and its metabolites are a group of compounds with thiol groups. Due to their reactivity with, it is difficult to obtain a good peak shape by general HPLC system. So, thiol groups are often specifically protected with… to obtain a good peak shape or in some cases fluorescently labeled for highly sensitive analysis2). However, derivatization process is time-consuming, and there are concerns that large excesses of labeling reagents may desensitize the mass spectrometer. In this study, we develop a method for quantitative analysis of underivatized GSH and its metabolites using an Agilent 1290 Infinity II Bio LC with MP35N® material. MP35N® material has excellent chemical resistance, then can be used over a wide pH range and salt concentration. Therefore, it is expected to be more inert to thiol groups than stainless steel (SUS), which is commonly used in general LC.