演題概要

オーラルセッション

第3日　6月24日（金）　10:20～10:40　D会場（413，414）

3D-O1-1020　　PDF

GC/MS calibration-curve-locking database (CCLD)を用いたヒト血漿サンプル半定量メタボロミクスワークフローの開発

(¹アジレント・ ²住友化学・ ³名大院医・ ⁴東農大農・ ⁵水産研・ ⁶九大生医研)
^o姉川彩¹・ 岩橋福松²・ 上山純³・ 辻井良政⁴・ 羽野健志⁵・ 秦康祐⁶・ 相馬悠希⁶・ 山下俊幸⁶・ 和泉自泰⁶・ 馬場健史⁶

We developed the semi-quant metabolomics workflow, which includes about 90 calibration curves for human plasma samples by GC/MS. In this study, we examined the possibility of semi-quantification screening using a calibration-curve-locking database (CCLD) for the concentration of hydrophilic metabolites in human plasma, NIST SRM1950, without requiring calibration curve as compared to 2019 and 2021 data sets. As a result of these examinations, the workflow and semi-quantification we developed using CCLD methods suggest to be effective in metabolomics for human plasma samples.