演題概要

ポスター発表

第2日　6月23日（木）　　P会場（501，502，503）

2P-42（2D-O1-1045）　　PDF

血液メタボロミクスデータ統合に向けたLC/MS分析法の定量性評価

(¹九大院シス生・ ²九大生医研・ ³学振PD)
^o今戸優理¹・ 高橋政友²・ 相馬悠希^1,2・ 油屋駿介^2,3・ 中谷航太²・ 和泉自泰^1,2・ 馬場健史^1,2

Metabolomics, mass spectrometry-based comprehensive analysis of metabolome, has attracted attention as an analytical approach to provide novel insight into metabolism and to search for biomarkers. However, one of the current problems in the field of metabolomics is the difficulty in integrating data collected using different equipment at different facilities, because many metabolomic methods have been developed independently and are unique to each laboratory. Herein, we evaluated the quantitative performance of four types of liquid chromatography (LC), including hydrophilic-interaction LC (HILIC), ion chromatography with an anion exchange column (AEX), LC with pentafluorophenylpropyl column (PFPP-LC), and unified HILIC/AEX, coupled with benchtop quadrupole Orbitrap high-resolution mass spectrometry for hydrophilic metabolome analysis of human plasma.