日本質量分析学会 第70回質量分析総合討論会会

演題概要

ポスター発表

第2日 6月23日(木)  P会場(501,502,503)

細胞外小胞のサブクラス化に向けたグライコプロテオームプロファイリング

(1京大院薬2医薬基盤健栄研3京大院工)
o金尾英佑1,2和田俊太郎3久保拓也3大塚浩二3秋吉一成3足立淳1,2石濱泰1,2

In this study, we focus on the specific separation of extracellular vesicles (EVs) by recognizing glycans on their surfaces. As a typical separation based on glycans, lectin affinity chromatography (LAC), which is currently used to purify glycoproteins, is usually employed. However, the pore size of the LAC packing material is relatively small, so the use of such materials is not suitable for the separation of EVs having over 100 nm in diameter. To achieve an effective EV separation, a spongy-like monolith (SPM), which consists of poly(ethylene-co-glycidyl methacrylate) (PEGM), is expected as a separation medium for LAC. In this study, we prepared two kinds of LAC columns made of the SPMs, immobilized with Sambucus sieboldiana lectin (SSA) or Concanavalin A (ConA). As a result, glycoproteins and liposomes effectively interacted with their respective lectins. Finally, the lectin-immobilized SPMs were employed to separate EVs, and then their different proteome profiles were determined by LC/MS/MS. These results demonstrate the heterogeneity of EVs based on their surface glycans.