ポスター発表
- 第2日 6月23日（木） P会場（501，502，503）
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2P-19 PDF
IP-MALDI-MSを用いた血漿Neurogranin分析法の開発
Neurogranin (Ng) is a cerebrospinal fluid (CSF) biomarker for synaptic dysfunction at Alzheimer’s disease (AD). The clinical significance of Ng peptides in plasma has remained unclear. In this study, we developed an immunoprecipitation combined with matrix-assisted laser desorption ionization time-of-flight mass spectrometry (IP-MALDI-MS) for Ng peptides. Some peaks corresponding to a variety of Ng peptides and proteins were detected in a plasma sample. The accuracy in linear regression model showed within 80-120% in three Ng peptides. Intra assay precision of Ng peptides in plasma was less than 20%CV. IP-MALDI-MS enable the highly sensitive and simultaneous semi-quantitative measurement of multiple Ng peptides levels. It could be helpful in the analysis of plasma Ng peptides for AD biomarker.