演題概要

ポスター発表

第2日　6月23日（木）　　P会場（501，502，503）

2P-11　　PDF

北アフリカ棲息種サソリButhacus leptochelysの毒液に含まれるジスルフィド結合含有ペプチドの構造決定

(¹京大院農・ ²Al-Azhar Univ.)
田中柾人¹・ Abdel-Wahab, Mohammed²・ Sarhan, Moustafa²・ ^o宮下正弘¹・ 中川好秋¹・ 宮川恒¹

The scorpion venom contains various components, in which peptides are main components responsible for the activities of the venom, such as insect toxicity. Most of these peptides contain multiple disulfide bonds, which often exhibit neurotoxicity. In this study, we searched for a peptide with multiple disulfide bonds from the venom of the North African scorpion Buthacus leptochelys and determined its structure using Edman degradation and MS/MS sequencing analysis. As a result, we could determine the sequence of a 66-residue peptide cross-linked by four disulfide bonds, which are similar to scorpion β-toxins.