ポスター発表
- 第2日 6月23日（木） P会場（501，502，503）
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2P-05 PDF
Steroid profiling of biological samples by LC-MS/MS
Steroid hormones are endogenous compounds that are synthesized from cholesterol by a series of enzymatic reactions in the adrenal cortex, gonads, and placenta. There are four classes of steroid hormones, which include corticosteroids, androgens, estrogens, and progestogens. The comprehensive analysis of those steroids in various biological samples has a significant impact on disease diagnosis or health monitoring. The aim of this study is to develop an efficient, sensitive and prompt analytical method for detecting steroid hormones in various biological samples such as blood, urine, tissues, and cerebrospinal fluid. However, detection of steroids, especially, those in urine, has a difficult issue, which owes to the conjugated steroids being involved with high contents. It thus prompted me to establish the analytical platform for profiling urinary steroids. I conducted optimization of sample pretreatment of urine, i.e., enzymatic hydrolysis, which could convert the conjugates to the free forms, removal of proteins, and purification by solid-phase extraction. The method allowed twenty-six steroids in total, after enzymatic hydrolysis, to be qualified or quantified at a time in human urine. The recovery of each steroids was no less than 80%.