演題概要

オーラルセッション

第2日　6月23日（木）　15:20～15:40　A会場（メインホール）

2A-O2-1520　　PDF

Nano-LC/MS/MSを基盤とした1細胞解析システムの開発

(¹九大生医研・ ²産総研健康医工学・ ³新大院医歯)
^o和泉自泰¹・ 秦康祐¹・ 中谷航太¹・ 山村昌平²・ 松本雅記³・ 馬場健史¹

Analytical methods to accurately identify and quantify comprehensive metabolites or proteins from single mammalian cells are still in development. The aim of this study was to develop a single-cell metabolomic and proteomic analytical system based on highly sensitive nano-liquid chromatography tandem mass spectrometry (nano-LC/MS/MS). We firstly developed a method called in-line sample preparation for efficient cellular metabolomics or proteomics (ISPEC). By combining live single-cell sampling, ISPEC, and nano-LC/MS/MS, we successfully detected approximately 50 metabolites or 300 proteins from single cells. Our single-cell proteomic and metabolomic analytical system represents a potentially useful tool for in-depth studies focused on cell metabolism and heterogeneity.