ポスター発表
- 第1日 6月22日(水) P会場(501,502,503)
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1P-35 PDF
シアル酸結合様式特異的誘導体化法を用いた超百寿者の血漿N-グライコプロテオミクス
Semi-supercentenarians (SSCs, older than 105 years) are a good example of human longevity, therefore physiological and biochemical analyses of SSCs can contribute to the understanding of healthy human aging. In this study, we performed N-glycoproteomics of plasma proteins obtained from SSCs and younger control groups using a combination of LC-MS/MS and sialic acid linkage-specific alkyl amidation (SALSA) method to discriminate between α2,3- and α2,6-sialic acid. As a result of LC-MS/MS analyses, 1676 N-glycopeptides from 91 proteins were identified and quantified. Among them, 366 N-glycopeptides were altered in SSCs compared to younger control groups. Furthermore, multivariate analysis identified 10 N-glycopeptides which were characteristic of SSCs. Most of the identified N-glycopeptides were derived from α1-acid glycoprotein 1.