日本質量分析学会 第70回質量分析総合討論会会

演題概要

ポスター発表

第1日 6月22日(水)  P会場(501,502,503)

シアル酸結合様式特異的誘導体化法を用いた超百寿者の血漿N-グライコプロテオミクス

(1都健康長寿医セ研2島津3慶大4阪大)
o津元裕樹1西風隆司2新井康通3池邉一典4石崎達郎1神出計4権藤恭之4増井幸恵1梅澤啓太郎1川上恭司郎1広瀬信義3早川禎宏2三浦ゆり1遠藤玉夫1

Semi-supercentenarians (SSCs, older than 105 years) are a good example of human longevity, therefore physiological and biochemical analyses of SSCs can contribute to the understanding of healthy human aging. In this study, we performed N-glycoproteomics of plasma proteins obtained from SSCs and younger control groups using a combination of LC-MS/MS and sialic acid linkage-specific alkyl amidation (SALSA) method to discriminate between α2,3- and α2,6-sialic acid. As a result of LC-MS/MS analyses, 1676 N-glycopeptides from 91 proteins were identified and quantified. Among them, 366 N-glycopeptides were altered in SSCs compared to younger control groups. Furthermore, multivariate analysis identified 10 N-glycopeptides which were characteristic of SSCs. Most of the identified N-glycopeptides were derived from α1-acid glycoprotein 1.