ポスター発表
- 第1日 6月22日（水） P会場（501，502，503）
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1P-09 PDF
プロテオミクスでの、QTOFのDutyCicleを向上させる技術を用いたDIAの利点
The ability to identify and quantify large number of proteins and peptides is of great importance in translational medicine and life science research. Data independent acquisition (DIA) approaches have been shown to surpass data dependent acquisition (DDA) methodologies in terms of protein identifications in complex matrices especially at shorter acquisition speeds. Our new QTOF system equipped with a novel Zeno trap(1 is able to deliver sensitivity gains in variable window SWATH acquisition. The built-in Zeno trap increases duty cycle at MS/MS level to over 90%, allowing for unprecedented gains in sensitivity (5-20x) at MS/MS, resulting in more identifications using Zeno DIA acquisition. We evaluated increases in protein and peptide identifications using SWATH acquisition vs. Zeno DIA at various EvoSep sample throughputs.