ポスター発表
- 第1日 6月22日（水） P会場（501，502，503）
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1P-05 PDF
臨床プロテオミクスを目指した炭酸ナトリウム沈殿法を用いて調製した乾燥ろ紙血由来のタンパク質の簡便かつ高感度な解析法の開発
Dried blood spots (DBS) are widely used for screening biomolecular profiles, including enzymatic activities. However, detection of minor proteins in DBS by liquid chromatography–mass spectrometry (LC-MS/MS) without pre-enrichment remains challenging because of the co-existence of large quantities of hydrophilic proteins. In this study, we address this problem by developing a simple method using sodium carbonate precipitation (SCP). SCP enriches hydrophobic proteins from DBS, allowing substantial removal of soluble proteins. In combination with SCP, we used quantitative LC-MS/MS proteome analysis in a data-independent acquisition mode to enhance the sensitivity and quantification limits of proteome analysis. As a result, identification of 1977 proteins in DBS is possible, including 585 disease-related proteins listed in the Online Mendelian Inheritance in Man.