オーラルセッション
- 第1日 6月22日（水） 18:10～18:30 A会場（メインホール）
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1A-O1-1810 PDF
高効率ペプチド抽出法を基盤としたペプチドミクス
The differential solubilization (DS) method that we developed enables high-efficiency extraction of peptides from serum/plasma, including peptides bound to carrier proteins such as albumin. Using this method, we directly identified an authentic peptide hormone using LC-MS and various novel bioactive peptides. Furthermore, we modified the DS method to make it applicable to tissue samples. The modified DS method achieved highly efficient and reproducible peptide extraction from a few milligrams of frozen tissue. Subsequent LC-MS/MS analysis successfully resulted in the identification of numerous authentic bioactive peptides and a novel bioactive peptide from animal tissues. The method based on our peptide extraction technique may facilitate the discovery of novel bioactive peptides and peptides that are related to disease.