日本質量分析学会 第70回質量分析総合討論会会

演題概要

オーラルセッション

第1日 6月22日(水) 16:30~16:50 A会場(メインホール)

イオンモビリティスペクトロメトリーによる新次元ペプチド分離

(1京大院薬・ 2Univ. of Manitoba・ 3医薬基盤健栄研)
o小形公亮1・ 張智翔1・ Yeung, Darien2・ Spicer, Victor2・ Krokhin, Oleg2・ 石濱泰1,3

Trapped ion mobility spectrometry (TIMS) provides a new ion mobility separation axis in addition to retention time and m/z in shotgun proteomics experiments. In phosphoproteomics, TIMS separations have enabled more reliable identification and quantification of positional isomers. In isobaric quantification using tandem mass tag (TMT), TIMS can be used to reduce co-eluting ions by an average of 7.6%. Furthermore, the contribution of peptide amino-acid sequence to CCS has been investigated using a dataset of ~134,000 peptides analyzed by TIMS. In addition, we systematically characterized the CCS values of 4,433 pairs of mono-phosphopeptide and corresponding unphosphorylated peptide ions and found nearly one- third of the mono-phosphopeptide ions showed smaller CCS values than their unphosphorylated counterparts.