ポスター発表
- 第3日 5月17日（木） ポスター会場
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3P-41 PDF
腸内細菌叢減少マウスにおける脳関門と脳実質のプロテオーム変動の解明
Intestinal flora is revealed to be associated with a wide variety of diseases and are expected to be applied into new therapeutic development. The purpose of the present study was to investigate alterations of proteome at the brain barrier and parenchyma in intestinal bacteria reduction model mice. Quantitative proteome analysis of isolated brain capillaries, cortex and hippocampus were conducted by nanoLC-SWATH-MS. In the isolated brain capillaries, expression of tight junction proteins and pericyte protein were increased in intestinal bacteria reduced mice, suggesting that the tight junctions of the brain barrier were tighter in the model mice. A protein associated with Alzheimer’s disease decreased in hippocampus and another protein associated with schizophrenia increased in cerebral cortex of the intestinal bacteria reduced mice. Changes of these proteins are opposite direction against the CNS disease progression. These results suggest that variations in intestinal bacteria contribute to brain barrier function and progression of CNS diseases.