Abstract

Table of contents

Poster Presentations

Day 3, May 17（Wed.）　　Room P (Foyer, Room 1004-1007)

3P-48　　PDF

Detection of Different Distributions of Acetaminophen and its Metabolite at 10 µm Spatial Resolution by Atmospheric Pressure Matrix-assisted Laser Desorption Ionization Imaging Mass Microscope

(¹Hamamatsu Ikadaigaku, ²Preppers, ³Int. Mass Imaging Center)
^oMd Al Mamun^1,2, Muedur Rahman¹, Takumi Sakamoto^1,2, Ariful Islam^1,2, Soho Oyama¹, Md Mahamodun Nabi¹, Tomohito Sato¹, Tomoaki Kahyo¹, Yutaka Takahashi^1,2, Mitsutoshi Setou^1,3

Matrix-assisted laser desorption ionization-mass spectrometry imaging (MALDI-MSI) and desorption electrospray ionization (DESI)-MSI has attracted much attention in drug distribution studies. However, the typical highest spatial resolution of MALDI-MSI and DESI-MSI is 50 µm which is not enough to resolve complex tissues (brain, kidney, etc). Moreover, many drugs in tissues are poorly detected or undetectable by vacuum MALDI-MSI and DESI-MSI. For example, acetaminophen (APAP) and one of its major metabolites, APAP-Cysteine (APAP-CYS), cannot be detected by vacuum MALDI-MSI without derivatization. To this end, we aim at the high spatial mapping of this drug and its metabolite in tissues. For this purpose, we employed a newly developed atmospheric pressure-MALDI imaging mass microscope (iMScope™ QT, Shimadzu, Japan). Interestingly, iMScope QT was capable of imaging native APAP and APAP-CYS in mice kidneys at 25 µm and 10 µm spatial resolution. APAP was accumulated in the renal pelvis, while APAP-CYS showed characteristic distributions in the outer medulla and renal pelvis. Our study showed the capability of iMScope QT to detect APAP and APAP-CYS at high spatial resolution (up to 10 µm) which is fairly higher compared to other MALDI-MSI and DESI-MSI instruments.