The Mass Spectrometry society of Japan - The 71st Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lecture

Symposium Sessions (Day1, Day2, Day3)

Basic Sessions (Day1, Day2, Day3)

Young Researchers' Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Special Evening Sessions

Corporate Program

Poster Presentations

Day 3, May 17（Wed.）　　Room P (Foyer, Room 1004-1007)

3P-34　　PDF

Medium vacuum chemical ionization (MVCI) mass spectrometry (MS) coupled with supercritical fluid extraction (SFE)/supercritical fluid chromatography (SFC) and application to biological sample analysis.

(1FRC/Osaka Univ., 2Kansai Univ.)
oToshinobu Hondo1, Chihiro Ota2, Yumi Miyake1, Michisato Toyoda1

Online coupling of supercritical fluid extraction (SFE) and supercritical fluid chromatography (SFC) with medium vacuum chemical ionization (MVCI) mass spectrometry (MS) has been investigated. This novel analytical method has great potential to quantitate lipophilic small molecules contained in an extremely small sample, such as a cell. Obtained lower limit of quantitation (LLOQ) for arachidonic acid and arachidic acid were 1.2 and 2.7 fmol, respectively. Sixteen free fatty acids were quantified from 100 nL human pool serum, with a repeatability of 0.1 to 16% of relative standard deviation.