Symposium Sessions (Day1, Day2, Day3)
Basic Sessions (Day1, Day2, Day3)
Young Researchers' Sessions (Day1, Day2, Day3)
Poster Presentations
- Day 3, May 17（Wed.） Room P (Foyer, Room 1004-1007)
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3P-22 PDF
Lipid Isomer Separation Using Travelling Wave Cyclic Ion Mobility Mass Spectrometry
The analysis and structural characterization of lipids remain challenging due to the chemical structural diversity and isomeric nature of lipids. The Cyclic ion mobility has a unique multi-pass scalable capability, that increases ion mobility resolution to meet a give challenge. Different lipid class standards were obtained from Avanti Polar Lipids with positional isomer, different double bond positions, cis and trans isomers, glucosyl and galactosyl ceramide isomers, phosphatidyl-mono, di, tris-phosphates and ganglioside isomers were investigated. The standard lipids were infused into the Cyclic IMS system. First, individual standards were analyzed to determine their arrival time distribution and then an equimolar mixture of the standards were analyzed. The results show that some of the isomers were baseline separated only after 5 passes. However other lipid isomers remained unresolved after even 50 passes mainly due to their structural similarity of the lipid isomers in the electrostatic field. In summary, the correct identification of lipids is critical in understanding their biological role and importance. With its unique multi-pass cyclic ion mobility capability, it is possible to scale ion mobility resolution to separate lipid isomers. Advanced modes of operation with ion activation followed by ion mobility separation offers new insights into lipid structural characterization.