Symposium Sessions (Day1, Day2, Day3)
Basic Sessions (Day1, Day2, Day3)
Young Researchers' Sessions (Day1, Day2, Day3)
Poster Presentations
- Day 3, May 17（Wed.） Room P (Foyer, Room 1004-1007)
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3P-21 PDF
Visualization of glutamic acid decarboxylase reaction in mouse brain using mass spectrometry imaging
Glutamic acid decarboxylase (GAD) is an enzyme that catalyzes the reaction that produces γ-aminobutyric acid (GABA) from glutamic acid. GAD is an important enzyme that regulates the amount of glutamic acid and γ-aminobutyric acid, which control neurotransmission in the brain. Furthermore, it has been reported that GAD concentration was decreased in the brains of stressed rabbits, suggesting a relationship between stress and GAD. Understanding the brain regions associated with the GAD response is expected to provide insight into the regions that respond to stress and the interrelationships among these regions under stressful conditions. However, there is no established method to investigate the localization of the GAD response in complex animal brain structures.
Enzyme histochemistry is the method that can visualize enzymatic reactions on tissues while maintaining tissue morphology. Recently, Mass spectrometry imaging (MSI) which is a technique to reveal the positional distribution of target molecules has been applied to enzyme histochemistry.
Although the method to visualize the GAD reaction has been established only in plants such as legume, there are no study of its application to animal tissues. In this study, I aimed to develop a method to visualize the localization of the GAD reaction using animal tissue, mouse brain.