Abstract

Poster Presentations

Day 3, May 17（Wed.）　　Room P (Foyer, Room 1004-1007)

3P-18　　PDF

Identification and quantification of disaccharides using ultraviolet photodissociation

(OMU)
^oDaiya Nagai, Akimasa Fujihara

Cold gas-phase hydrogen-bonded clusters of tryptophan (Trp) enantiomers and disaccharide isomers were generated by electrospray ionization and collisional cooling in an ion trap at 8 K. Product ion spectra in the 265–290 nm wavelength range were obtained using tandem mass spectrometry. The photoreactivity differed between the enantiomers and isomers contained in the clusters at the photoexcitation of 278 nm. A calibration curve for the quantification of disaccharide isomers in solution was constructed by photoexcitation of the hydrogen-bonded clusters of disaccharide isomers with H+(L-Trp) at 278 nm.