Symposium Sessions (Day1, Day2, Day3)
Basic Sessions (Day1, Day2, Day3)
Young Researchers' Sessions (Day1, Day2, Day3)
Poster Presentations
- Day 3, May 17（Wed.） Room P (Foyer, Room 1004-1007)
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3P-16 PDF
Application of LC-MS/MS with FAIMS Pro Duo interface for highly selective and sensitive peptide screening method in equine urine
The use of peptide drugs is completely banned at any levels in horseracing and equestrian sports. Owing to the expected low concentrations of peptide drugs and/or their metabolites in complex equine biological matrices, it’s necessary for a doping control laboratory to develop a sensitive and specific test method for the detection of this class of substances. The authors’ laboratory has long been using liquid chromatography/tandem quadrupole mass spectrometry (LC-MS/MS) for the detection of peptides in equine urine and plasma, however, serious matrix influences were commonly observed for the detection of peptides at sub-ppb levels. In this presentation, a highly selective and sensitive screening method for the detection of over 35 peptides at low pg/mL levels in equine urine was developed using ThemoFisher Scientific Vanquish Flex UHPLC coupled to TSQ Quantis Plus equipped with a high-field asymmetric waveform ion mobility spectrometry (FAIMS) interface. Our results showed that the FAIMS Pro Duo interface could significantly reduce the background chemical noise and co-eluting matrix interferences, leading to improved detection selectivity. With this newly-developed method, the limits of detection (LODs) for some of the targets could reach down to less than 2 pg/mL. Optimization of sample extraction method and validation results will be presented.