The Mass Spectrometry society of Japan - The 71st Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lecture

Symposium Sessions (Day1, Day2, Day3)

Basic Sessions (Day1, Day2, Day3)

Young Researchers' Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Special Evening Sessions

Corporate Program

Poster Presentations

Day 3, May 17（Wed.）　　Room P (Foyer, Room 1004-1007)

3P-14　　PDF

Proximity Corona Discharge Ion Source Applied to the Analysis of Facial Lipids: Matrix-Effect Free APCI

(1BioChromato, 2Univ. Yamanashi, 3Johns Hopkins Univ.)
oHaruo Shimada1, Kazumasa Kinoshita1, Satoshi Ninomiya2, Kenzo Hiraoka2, Stephanie Rankin-Turner3

In heat pulse desorption (HPD)/mass spectrometry, a heated N2 gas pulse was directed at the sample surface to desorb the analytes and then instantaneously introduced into the mass spectrometer via a corona discharge ion source. As a result, oxygen-containing and nitrogen-containing compounds were detected regardless of their proton affinities. This indicates that successive proton-transfer reactions to form protonated compounds which have high proton affinities do not take place in the APCI ion source, characterized by the proximity of the needle tip to the MS inlet, used in this work. The lifetime of the exoergic proton transfer reactions for analytes with a concentration of 1 ppm is calculated to be about 30μs. Thus, the reactions were estimated to take place only once in the proximity space between the needle tip and the MS inlet. Under these conditions, analytes could be detected almost quantitatively. In this work, HPD was applied to skin surface lipids collected on a cotton bud. Cholesterol, squalene, wax ester, ceramides, TAG in addition to all essential amino acids and other components were detected. We also discuss the results for linearity of fatty acids using this APCI ion source.