The Mass Spectrometry society of Japan - The 71st Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lecture

Symposium Sessions (Day1, Day2, Day3)

Basic Sessions (Day1, Day2, Day3)

Young Researchers' Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Special Evening Sessions

Corporate Program

Young Researchers' Sessions

Day 3, May 17（Wed.）　10:00-10:15　Room C (Room 1009)

3C-O-1000（2P-17）　　PDF

Spatial Proteome Imaging by Machine-gun Proteomics

(1Kyoto Univ., 2RIKEN IMS, 3Keio Univ., 4NIBIOHN)
oAyana Tomioka1, Ryota Tomioka1, Kosuke Ogata1, Issei Mori2,3, Makoto Arita2,3, Koshi Imami2, Naoyuki Sugiyama1, Yasushi Ishihama1,4

Spatial proteomics is a promising technique to profile protein expression in different tissue regions. While current antibody-based spatial mapping methods are sensitive, selective, and high-resolution, they can quantify only pre-defined proteins. In contrast, shotgun proteomics enables untargeted protein quantitation and has the potential to break through the limitation of antibody-based methods. However, typical shotgun proteomics requires several hours per nanoLC/MS/MS measurement, making the combination with MS imaging impractical. We have therefore developed a “machine-gun proteomics” platform that is more than 100 times faster than typical shotgun proteomics, at only 1.4 minutes per sample. Our method could obtain 1,000 sections within a day, allowing us to obtain not only 2D, but 3D spatial proteome expression profiles within a feasible time.