Abstract

Young Researchers' Sessions

Day 3, May 17（Wed.）　09:30-09:45　Room C (Room 1009)

3C-O-0930（1P-03）　　PDF

Biomarker discovery of lung adenocarcinoma using extracellular vesicles derived from human plasma by LC/MS

(¹Teikyo Univ., ²Teikyo Univ., ³Jpn. Fdn. Cancer Res., ⁴Tohoku Univ., ⁵Nippon Boehringer Ingelheim Co., Ltd, ⁶Teikyo University School of Medicine Mizonokuchi Hospital)
^oYuko Iwasaki¹, Takeshi Honda², Koji Ueda³, Tomoko Fukuuchi¹, Noriko Yamaoka¹, Eiji Hishinuma⁴, Naomi Matsukawa⁴, Akira Togashi⁵, Noriyuki Matsutani⁶, Nobuhiko Seki², Daisuke Saigusa^1,4

Extracellular Vesicles (EVs) contain metabolites, such as nucleotides and lipid species, and transport them playing as the intracellular mediators. However, it is hard to detect these metabolites due to the limit of detection. We firstly established the analytical procedure for metabolic profiling of EVs by means of UHPLC-MS/MS and UHPLC-FTMS analysis. Then, we examined to identify the metabolic variation in plasma EVs derived from lung adenocarcinoma patients and healthy volunteers. As results, cysteine and cystine were significantly higher in the EVs from cancer patient, and observed the variation of lipid species, such as triglycerides and phosphatidylcholines consist with polyunsaturated fatty acids. We surmise that the oxidative stress related metabolites in EVs might be correlated with the progression of cancer, and the mechanism should be elucidated in the future study.