Abstract

Basic Sessions

Day 3, May 17（Wed.）　09:51-10:09　Room B (Room 1202)

3B-O-0951　　PDF

An alternative method for quantitative mass spectrometry imaging (q-MSI) of dopamine utilizing fragments of animal tissue

(¹Osaka Univ., ²Miruion Inc)
^oShuichi Shimma^1,2, Erika Nagano², Kazuki Odake²

Mass Spectrometry Imaging (MSI) is a well-known method for the ionization of molecules on tissue sections and the visualization of their localization. Recently, different sample preparation methods and new instruments have been used for MSI, and different molecules are becoming visible. On the other hand, although several quantification methods have been proposed, there is still room for the development of a simplified procedure. Here, we have attempted to develop a reproducible and reliable quantification method using a calibration curve prepared from tissue debris of a frozen section of a sample when we trim the frozen blocks. In this study, we aimed to establish a reproducible and reliable quantification method using a calibration curve derived from tissue fragments of a frozen section sample. We discuss the reproducibility of this method across different sample lots and the effect of the biological matrix (ion suppression) on our results. The quantitative performance was evaluated in terms of accuracy and precision, and the reliability of the quantitative values obtained by MALDI-MSI was further evaluated by ELISA. Our q-MSI method for the quantification of dopamine in mouse brain tissue was found to be highly linear, accurate, and precise. The quantitative values obtained by MSI were found to be highly comparable (>85% similarity) to the results obtained by ELISA from the same tissue extracts.