The Mass Spectrometry society of Japan - The 71st Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lecture

Symposium Sessions (Day1, Day2, Day3)

Basic Sessions (Day1, Day2, Day3)

Young Researchers' Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Special Evening Sessions

Corporate Program

Symposium Sessions

Day 3, May 17（Wed.）　14:37-14:59　Room A (Special Conference Room)

3A-S-1437　　PDF

Co-Fractionation Mass Spectrometry analysis of very large cellular protein complexes

(Tokushima Univ.)
oHarunori Yoshikawa

Several different methods have been developed to analyze protein complexes. In addition to Affinity-Purification (AP)-MS and proximity labelling approaches, e.g. BioID and APEX, Co-Fractionation Mass Spectrometry (CF-MS) analysis has been recently introduced for the systematic characterization of cellular protein complexes. Using uHPLC-based Size Exclusion Chromatography (SEC), we have shown that even very large cellular complexes, including ribosomes and polysomes, can be separated efficiently by uHPLC using SEC columns with a pore size of 2,000Å. Here, we apply this technique to isolate and characterize intact nucleolar pre-ribosomal particles from human cells.