The Mass Spectrometry society of Japan - The 71st Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lecture

Symposium Sessions (Day1, Day2, Day3)

Basic Sessions (Day1, Day2, Day3)

Young Researchers' Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Special Evening Sessions

Corporate Program

Poster Presentations

Day 2, May 16（Tue.）　　Room P (Foyer, Room 1004-1007)

2P-43　　PDF

Interlaboratory study of Multi-Attribute Method (MAM) for Antibody Therapeutics - Post-Translational Modification (3) C-terminal Lys Clipping -

(1Taiho Pharmaceutical, 2Astellas Pharma, 3Shionogi, 4Daiichi-Sankyo, 5Eisai, 6Sawai Pharmaceutical, 7Chugai Pharm., 8NIMS, 9Univ.Tsukuba)
oYuta Kobayashi1, Naoki Kawase2, Masaaki Imono3, Takahiro Kogaki4, Mami Gomibuchi5, Masaaki Senda6, Maki Yoshida7, Kohsaku Kawakami8,9

The Multiple Attribute Method (MAM), based on peptide mapping by LC/MS2 analysis, is an analytical method that provides a better understanding of the quantitative evaluation of antibody drug heterogeneities.
The National Institute for Materials Science (NIMS) and 11 pharmaceutical companies in Japan have established a Materials Open Platform (MOP) as a Center of Excellence (COE) in 2021, which aims to improve the infrastructure of research and development and standardize development methods. The Antibody Analysis Group, one of the groups in the COE, is organized to develop the MAM in Japan.
In this presentation, we will present our findings from the analysis of C-terminal Lys-Clipping in the experiment. The mass spectra obtained by MAM are generally detected as Multi-charged ions. The results of the Lys-Clipping analysis suggest that the variation of the quantification value among companies may be reduced by matching the charge status of the peptides for which ion intensities are calculated for Clipping and non-Clipping peptides when calculating the percentage of Lys-Clipping.