The Mass Spectrometry society of Japan - The 71st Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lecture

Symposium Sessions (Day1, Day2, Day3)

Basic Sessions (Day1, Day2, Day3)

Young Researchers' Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Special Evening Sessions

Corporate Program

Poster Presentations

Day 2, May 16（Tue.）　　Room P (Foyer, Room 1004-1007)

2P-20　　PDF

Development of separation method for hexosylceramides by time-of-flight ion mobility mass spectrometry

(1Teikyo Univ., 2Univ. Tokyo)
oRina Inoue1, Kuniyuki Kano2, Tomoko Fukuuchi1, Noriko Yamaoka1, Junken Aoki2, Daisuke Saigusa1

Glucosylceramide (GlcCer) is regulated by glucocerebrosidase (GBA) and essential for maintaining the biological homeostasis. The enzymatical activity of GBA was related with the expression of disease, such as Gaucher. However, galactosylceramide (GalCer), which is the isomeric component of GlcCer must be separated to evaluate the accurate GBA activity. We therefore examined to separate GlcCer and GalCer by means of ion mobility mass spectrometry (IMS) technique using cyclic IMS to develop the analytical method of GlcCer and GalCer in biological samples. As the result, the 50 passes enabled us to have a good separation (Rs = 0.7) of GlcCer (d18:1/18:0) and GalCer (d18:1/18:0) by infusion analysis of standard solutions (1 mg/mL) by cyclic IMS. The optimal condition would be applied for the LC-cyclicIMS-TOF/MS and DESI-MS imaging analyses.