The Mass Spectrometry society of Japan - The 71st Annual Conference on Mass Spectrometry, Japan

Abstract

Poster Presentations

Day 2, May 16(Tue.)  Room P (Foyer, Room 1004-1007)

Quantitative analysis of O-GlcNAcylated proteins in cells by data-independent acquisition mass spectrometry

(Yokohama City Univ.)
oShunsuke Hoshina, Takaya Urasawa, Rika Nshimura, Daisuke Takakura, Nana Kawasaki

An O-GlcNAcylated peptide spectral library was created by data-dependent acquisition mass spectrometry of O-GlcNAcylated peptides from human induced pluripotent stem cells (hiPSC). Using the library, we performed data-independent mass spectrometry of iPSC and iPSC-derived neural stem cells and neuron. O-GlcNAcylation of host cell factor 1 was found to be altered during neuronal differentiation.